Education

PhD, University of California, Santa Cruz
BS, Loyola University of Chicago

Research Interests

The central aim of my research is to develop methodologies and “rules” for enzyme design, and apply these methods to efficiently create novel and practical biocatalysts. My current research interest is in engineering proteins using directed evolution and rational approaches. My research goals include developing high-throughput screening technologies to assay mutant enzyme libraries to discovery novel biocatalyst, combine rational and directed evolution approaches to create de novo enzymes, metabolic pathway engineering in bacteria, novel protein display technologies, and incorporation of nonnatural amino acids in proteins.

Selected Recent Publications

Alcalde, M.*,  Farinas, E.*, and Arnold, F (2004).  "Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450BM-3 variant 139-3,"  Journal of Biomolecular Screening,  9, 141-146. * These authors contributed equally.

Farinas, E., Alcalde, M., and Arnold, F. (2003).  "Alkene epoxidation catalyzed by cytochrome P450 BM-3  139-3,"  Tetrahedron, 60, 525-528.

Glieder, A.*, Farinas. E.*, and Arnold, F. (2002).  "Laboratory Evolution of a soluble, self-sufficient, highly active alkane hydroxylase,"  Nature Biotechnology, 20, 1135-1139.

* These authors contributed equally.

Farinas, E., Schwaneberg, U., Glieder, A., and Arnold, F. (2001). "Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation,"  Advanced Synthesis and Catalysis, 343, 601-606.

Schwaneberg, U., Otey, C., Cirino, P., Farinas, E., and Arnold, H. (2001). "Cost-effective Whole-cell assay for Laboratory Evolution of Hydroxylases in Escherichia coli," Journal of Biomolecular Screening, 6, 111-118.