You are in the College of Science and Liberal ArtsCollege of Science and Liberal Arts

Department of Chemistry and Environmental Science

Farinas, Edgardo T.

Contact Info
Title: Associate Professor
Email: edgardo.t.farinas@njit.edu
Office: 151A tiernan
Phone: 973-642-7363
Dept: Chemistry and Environmental Science
Webpage: http://web.njit.edu/~edgardo

Academic Interests: enzyme design, biocatalysts, proteins

About Me

Edgardo T. Farinas, PhD, is an assistant professor in the department of chemistry and environmental science at New Jersey Institute of Technology. The central aim of his research is to develop methodologies and “rules” for enzyme design, and apply these methods to efficiently create novel and practical biocatalysts. His current research interest is in engineering proteins using directed evolution and rational approaches. His research goals include developing high-throughput screening technologies to assay mutant enzyme libraries to discovery novel biocatalyst; combining rational and directed evolution approaches to create de novo enzymes; metabolic pathway engineering in bacteria; novel protein display technologies; and incorporation of nonnatural amino acids in proteins.

In 2008, Farinas received a National Science Foundation (NSF) CAREER award for his project "New Tools for High-Throughput Screening of Protein Libraries: Engineering Metalloproteins Displayed on Bacillus Subtilis Spores." The prestigious career award recognizes teacher-scholars most likely to become the academic leaders of the 21st century.

Education

  • PhD, University of California, Santa Cruz
  • BS, Loyola University of Chicago

Courses I Teach

DOCT DISSERTATION & RES
BIOCHEMISTRY LAB I
RESEARCH & INDEP STUDY I
INDEPENDENT STUDY I
PRE-DOCTORAL RESEARCH
MASTERS PROJECT
MASTERS THESIS
MASTERS THESIS
DOCTORAL DISSERTATION
DOCTORAL DISSERTATION
DOCTORAL DISSERTATION
DOCTORAL DISSERTATION
GRADUATE WORK EXPERIENCE
GRADUATE WORK EXPERIENCE
GRADUATE CO-OP WORK EXP IV
INDEPENDENT STUDY I
MASTERS PROJECT
DOCTORAL DISSERTATION
DOCT DISSERTATION & RES
DOCT DISSERTATION & RES
DOCT DISSERTATION & RES
PRE-DOCTORAL RESEARCH

Research Interests

The central aim of my research is to develop methodologies and “rules” for enzyme design, and apply these methods to efficiently create novel and practical biocatalysts. My research goals include developing high-throughput screening technologies to assay mutant enzyme libraries to discovery novel biocatalyst, combine rational and directed evolution approaches to create de novo enzymes, metabolic pathway engineering in bacteria, novel protein display technologies, and incorporation of nonnatural amino acids in proteins.

Current Research

My current research interest is in engineering proteins using directed evolution and rational approaches.


Refereed Journal Articles

12. Miguel Alcalde*, Edgardo T. Farinas*, Frances H. ArnoldColorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450BM-3 variant 139-3Journal of Biomolecular Screening, 2004,  9, 141-146.

*These author contributed equally 

11. Alkene epoxidation catalyzed by cytochrome P450 BM-3  139-3.  Edgardo T. Farinas, Miguel Alcalde, and Frances Arnold, Tetrahedron, 2003, 60, 525-528.

10. Laboratory Evolution of a soluble, self-sufficient, highly active alkane hydroxylase.  Anton Glieder*, Edgardo T. Farinas*, Frances H. Arnold.  Nature Biotechnology,  2002, 20, 1135-1139.

*These author contributed equally 

9.  Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation. Edgardo T. Farinas, Ulrich Schwaneberg, Anton Glieder, Frances H. Arnold. Advanced Synthesis and Catalysis, 2001, 343, 601-606.

8.  Cost-effective Whole-cell assay for Laboratory Evolution of Hydroxylases in Escherichia coli.  Ulrich Schwaneberg, Christopher Otey, Patrick C. Cirino, Edgardo Farinas, and Frances H. Arnold. Journal of Biomolecular Screening, 2001, 6, 111-118.  

7.  The de novo design of a Rubredoxin-like Fe site.  Edgardo T. Farinas and Lynne Regan. Protein Science,  1998, 7, 1939-1946. 

6.  Photoinduced Oxidation of Hydrocarbons with Cobalt(III)-alkylperoxy Complexes. Edgardo T. Farinas, Cattien Nguyen, and Pradip K. Mascharak. Inorganica Chimica Acta,1997, 263, 17-21. 

5.  Photoinduced DNA Cleavage Reactions by Designed Analogues of Co(III)-Bleomycin, The Metalated Core is the Primary Determinant of Sequence Specificity.  Edgardo T. Farinas, Jennifer D. Tan, and Pradip K. Mascharak.  Inorganic Chemistry, 1996, 35, 2637. 

4.  Structural Features that Control Oxygen Activation at the Non-Heme Iron Site in Fe(II)-Bleomycin:  An Analogue Study.  Richard J. Guajardo, Ferman Chavez, Edgardo T. Farinas, and Pradip K. Mascharak.  Journal of the American Chemical Society, 1995, 117, 3883.

3.  Electron Paramagnetic Resonance Studies on the Formation and Decomposition of the Oxygenated Product of [CoII(PMA)]+, a Synthetic Analogue of Co(II)-Bleomycin. Edgardo T. Farinas, Narayan Baidya and Pradip K. Mascharak.  Inorganic Chemistry 1994, 33, 5970. 

2.  NMR Evidence of Sequence Specific DNA Binding by a Co(III)-Bleomycin Analogue with Tethered Acridine.  Jennifer D. Tan, Edgardo T. Farinas, Sheila S. David, and Pradip K. Mascharak. Inorganic Chemistry , 1994, 33, 4295.

1.  A Designed Synthetic Analogue of Co(III)-Bleomycin with Enhanced DNA-Binding and Photocleaving Activity. Edgardo T. Farinas, Jennifer D. Tan, Narayan Baidya, and Pradip K. Mascharak. Journal of the American Chemical Society  1993, 115, 2996.

Patent Applied for

1. Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation. 07/20/01, Reference No. CIT-3514

Invited Articles/Book Chapters

4. Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted Alkanes.  Direct Evolution Methods  Book II: Methods in Molecular Biology Series. Eds.  F. H. Arnold and G. Georgiou. Humana Press, Totowa, New Jersey.  2003, 149-155.

3. Directed Enzyme Evolution. Edgardo T. Farinas, Thomas Bulter, Frances H. Arnold. Current Opinions in Biotechnology, 2001, 12, 545-551. 

2.  Syntheses, Structures and Reactivities of Designed Analogues of Cobalt(III)-Bleomycins:  Insight into the Mechanism of Sequence - specific DNA Cleavage upon Illumination.  Edgardo T. Farinas and Pradip K. Mascharak.  Proceedings of Indian Academy of Science (Chem. Soc.), 1995, 107, 459. 

1.  Structure and Reactivity of Fe(II)-SAr Complexes:  Relevance to the Active Site Of Isopenicillin N Synthase, Edgardo T. Farinas and Pradip K. Mascharak. ChemTracts.  1995, 7, 33.

Presentations

12.  “Laboratory Evolution of P450 BM-3 for Alkane Hydroxylation”.  Edgardo T. Farinas, Anton Glieder, Frances H. Arnold.  Sixth International Symposium on Cytochrome P450 Biodiversity. Los Angeles, CA August 20-25, 2002.

11.  “Laboratory Evolution of Cytochrome P450 BM-3 into an Efficient Alkane Hydroxylase”.  Edgardo T. Farinas, Anton Glieder, Frances H. Arnold. California Catalysis Society, Pasadena, CA October 24-25, 2001.

10. “Directed Evolution of Cytochrome P450 BM3: Design of an enzymatic system to make a diol from an alkane”.  Edgardo Farinas and Frances Arnold.  An oral presentation. The 2000 Conference of Ford Fellows (Academic  Exchange Session), Irvine, California, October 13-14, 2000.

 9.  “The de novo design of a Rubredoxin-like Fe site”. A poster presentation. The Protein Society 12th Symposium.  San Diego, CA July 25 - 29, 1998.

 8.  “Design of an Electron Transfer Metalloprotein” Edgardo Farinas and Lynne Regan. An oral presentation.  Chemical Biology Seminar Series. Yale University, New Haven CT, May 1, 1998.

 7.  “Design of de novo [Fe(Cys)­4]-1,-2 Sites in Proteins” Edgardo Farinas and Lynne Regan. Poster presentation. 1997 23rd Annual Symposium of the Department of Molecular Biophysics and Biochemistry. The Swope Center Marine Biological Laboratories, Woods Hole, October 17 -19, 1997

6.  “Photoinduced Alkane Functionalization with Mononuclear Cobalt(III) Alkylperoxy Complexes.”  Edgardo T. Farinas, Cattien Nguyen, and Pradip K. Mascharak. Poster presentation. 1996 Pacific Conference.  San Francisco, California.  October 29 - November 1, 1996 

5.  “In Photoinduced DNA Cleavage Reactions by Designed Analogues of Co(III)-Bleomycin, The Metallated Core is the Primary Determinant of Sequence Specificity.”  Edgardo T. Farinas and Pradip K. Mascharak.  An oral presentation by Pradip K. Mascharak for Mechanisms of Metal Mediated Biopolymer Cleavage a Symposium at the 212th American Chemical Society National Meeting.  Orlando, Florida.  August 25 - 27, 1996.

 4.  “In Photoinduced DNA Cleavage Reactions by Designed Analogues of Co(III)-Bleomycin, The Metallated Core is the Primary Determinant of Sequence Specificity.”  Edgardo T. Farinas, Jennifer D. Tan, and Pradip K. Mascharak. Poster presentation. Seventh International Conference on Bioinorganic Chemistry.  Lubeck, Germany.  September 3 - 8, 1995

 3. “Sequence-specific DNA Binding and Cleavage by a Co(III)-Bleomycin Analogue With Tethered Acridine.”  Edgardo T. Farinas, Jennifer D. Tan, Sheila S. David, and Pradip K. Mascharak. Poster presentation. 208th American Chemical Society National Meeting, Washington DC.  August 21 - 25, 1994.

 2.  “Electron Paramagnetic Resonance Studies on a Synthetic Analogue of Cobalt-Bleomycin.”  Edgardo T. Farinas, Narayan Baidya and Pradip K. Mascharak. Poster presentation. Sixth International Conference on Bioinorganic Chemistry.  University of California, San Diego.  August 23-27, 1993.

1.  “Enhanced DNA Photocleavage by a New Synthetic Analogue of Co(III)-Bleomycin.” Edgardo T. Farinas, Jennifer D. Tan, Narayan Baidya, and Pradip K. Mascharak. Poster presentation. 205th. American Chemical Society National Meeting. Denver, Colorado.   March 28- April 2, 1993.