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Edgardo Farinas

Farinas, Edgardo T.
Associate Professor, Chemistry and Environmental Science
386 Tiernan
About Me

Edgardo T. Farinas, PhD, is an assistant professor in the department of chemistry and environmental science at New Jersey Institute of Technology. The central aim of his research is to develop methodologies and “rules” for enzyme design, and apply these methods to efficiently create novel and practical biocatalysts. His current research interest is in engineering proteins using directed evolution and rational approaches. His research goals include developing high-throughput screening technologies to assay mutant enzyme libraries to discovery novel biocatalyst; combining rational and directed evolution approaches to create de novo enzymes; metabolic pathway engineering in bacteria; novel protein display technologies; and incorporation of nonnatural amino acids in proteins.

In 2008, Farinas received a National Science Foundation (NSF) CAREER award for his project "New Tools for High-Throughput Screening of Protein Libraries: Engineering Metalloproteins Displayed on Bacillus Subtilis Spores." The prestigious career award recognizes teacher-scholars most likely to become the academic leaders of the 21st century.

Education
  • Loyola University of Chicago (BS Chemistry 1990)
  • University of California, Santa Cruz (PhD Chemistry 1997); Advisor: Pradip Mascharak
  • Yale University (Protein Design; Postdoc 1997-1999); Advisor: Lynne Regan
  • California Institute of Technology (Protein Evolution; Postdoc 1999-2003); Advisor: Frances Arnold (Noble Prize Chemistry 2018)
  • University of Texas at Austin (FACS Screening; Postdoc 2003-2004); Advisor: George Georgiou and Brent Iverson
Research Interests

The central aim of my research is to develop methodologies and “rules” for enzyme design, and apply these methods to efficiently create novel and practical biocatalysts. My research goals include developing high-throughput screening technologies to assay mutant enzyme libraries to discovery novel biocatalyst, combine rational and directed evolution approaches to create de novo enzymes, metabolic pathway engineering in bacteria, novel protein display technologies, and incorporation of nonnatural amino acids in proteins.

Current Research

My current research interest is in engineering proteins using directed evolution and rational approaches.

Refereed Journal Articles

19. Chakraborty, J., Nemeria, N.S., Farinas, E., Jordan, F. Catalysis of transthioacylation in the active centers of dihydrolipoamide acyltransferase comomponets of 2-oxo acid dehydrogenase complex FEBS OpenBio , ∫2018, 8 (6) 880-896.

18. Han Jia, Frederick S. Lee, and Edgardo T. Farinas. Bacillus subtilis Spore Display of Laccase for Evolution under Extreme Conditions of High Concentrations of Organic Solvent, ACS Combinatorial Science, vol 16, 2014, no 12, 665-669. (peer)

17. Hetalben Patel, Da Jeong Shim, Edgardo T. Farinas, Frank Jordan Investigation of the donor and acceptor range for chiral carboligation catalyzed by the E1 component of the 2-oxoglutarate dehydrogenase complex.
Journal of Molecular Catalysis B: Enzymatic, vol 98, 2013, 42-45. (peer)

16. Da Jeong Shim, Natalia S. Nemeria, Anand Balakrishnan, Hetalben Patel, Jaeyoung Song, Junjie Wang, Frank Jordan, and Edgardo T. Farinas. Assignment of Function to Histidines 260 and 298 by Engineering the E1 Component of the Escherichia coli 2-Oxoglutarate Dehydrogenase Complex; Substitutions That Lead to Acceptance of Substrates Lacking the 5-Carboxyl Group. Biochemistry, vol 50, 2011,77057709, DOI: 10.1021/bi200936n. http://pubs.acs.org/journal/bichaw (peer).

15. Nirupama Gupta and Edgardo T. Farinas, Directed evolution of CotA laccase for increased substrate specificity using Bacillus subtilis spores. Protein Engineering, Design, & Selection, vol 23, no 8, 2010, 679-682 (peer)

14. Nirupama Gupta, Frederick S. Lee and Edgardo T. Farinas, Laboratory Evolution of Laccase for Substrate Specificity, Journal of Molecular Catalysis B: Enzymatic, vol 62, no 3-4 2009, 230-234 (peer)

13. Nirupama Gupta and Edgardo T. Farinas, Narrowing Laccase Substrate Specificity Using Active Site Saturation Mutagenesis, Combinatorial Chemistry and High Throughput Screening, vol 12, no 3, 2009, 269-274 (peer).

This manuscript is featured on the journal cover.

12. Miguel Alcalde*, Edgardo T. Farinas*, Frances H. Arnold. Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450BM-3 variant 139-3 Journal of Biomolecular Screening, vol 9, no 2, 2004, 141-146 (peer).

*These author contributed equally

11. Edgardo T. Farinas, Miguel Alcalde, and Frances Arnold. Alkene epoxidation catalyzed by cytochrome P450 BM-3 139-3. Tetrahedron, vol 60, no 3, 2003, 525-528 (peer).

10. Anton Glieder*, Edgardo T. Farinas*, Frances H. Arnold. Laboratory Evolution of a soluble, self-sufficient, highly active alkane hydroxylase. Nature Biotechnology, vol 20, no 11, 2002, 1135-1139 (peer).

*These author contributed equally

9. Edgardo T. Farinas, Ulrich Schwaneberg, Anton Glieder, Frances H. Arnold. Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation. Advanced Synthesis and Catalysis, vol 343, no 6-7, 2001, 601-606 (peer).

8. Ulrich Schwaneberg, Christopher Otey, Patrick C. Cirino, Edgardo Farinas, and Frances H. Arnold.Cost-effective Whole-cell assay for Laboratory Evolution of Hydroxylases in Escherichia coli. Journal of Biomolecular Screening, vol 6, no 2, 2001, 111-118 (peer).

7. Edgardo T. Farinas and Lynne Regan. The de novo design of a Rubredoxin-like Fe site. Protein Science, vol 7, no 9, 1998,1939-1946 (peer).

6. Edgardo T. Farinas, Cattien Nguyen, and Pradip K. Mascharak Photoinduced Oxidation of Hydrocarbons with Cobalt(III)-alkylperoxy Complexes. Inorganica Chimica Acta, vol 263, no 1-2, 1997, 17-21 (peer).

5. Edgardo T. Farinas, Jennifer D. Tan, and Pradip K. Mascharak. Photoinduced DNA Cleavage Reactions by Designed Analogues of Co(III)-Bleomycin, The Metalated Core is the Primary Determinant of Sequence Specificity. Inorganic Chemistry, vol 35, no 9, 1996, 2637-2543 (peer).

4. Richard J. Guajardo, Ferman Chavez, Edgardo T. Farinas, and Pradip K. Mascharak. Structural Features that Control Oxygen Activation at the Non-Heme Iron Site in Fe(II)-Bleomycin: An Analogue Study. Journal of the American Chemical Society, vol 117, no 13, 1995, 3883-3884 (peer).

3. Edgardo T. Farinas, Narayan Baidya and Pradip K. Mascharak. Electron Paramagnetic Resonance Studies on the Formation and Decomposition of the Oxygenated Product of [CoII(PMA)]+, a Synthetic Analogue of Co(II)-Bleomycin. Inorganic Chemistry vol 33, no 25, 1994, 5970-5973 (peer).

2. Jennifer D. Tan, Edgardo T. Farinas, Sheila S. David, and Pradip K. Mascharak. NMR Evidence of Sequence Specific DNA Binding by a Co(III)-Bleomycin Analogue with Tethered Acridine. Inorganic Chemistry , vol 33, no 19, 1994, 4295-4308 (peer).

1. Edgardo T. Farinas, Jennifer D. Tan, Narayan Baidya, and Pradip K. Mascharak.A Designed Synthetic Analogue of Co(III)-Bleomycin with Enhanced DNA-Binding and Photocleaving Activity Journal of the American Chemical Society, vol 115, no 7, 1993, 2996-2997 (peer).